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Conditional quantitative trait locus mapping of wheat seed protein-fraction in relation to starch content

机译:小麦种子蛋白质组分与淀粉含量的条件性状特征位点作图

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摘要

Protein and starch are important in wheat quality and yield. To understand the genetic relationship between protein and starch at the quantitative trait locus (QTL)/gene level, 168 doubled haploid (DH) lines were used at three locations over 2 years. The QTLs for proteinfraction contents and starch content were analyzed by unconditional and conditional QTL mapping. We detected 17 unconditional additive QTLs (four albumin QTLs, three globulin QTLs, six gliadin QTLs, four glutenin QTLs) controlling protein-fraction contents. We detected 19 conditional QTLs (five albumin QTLs, three globulin QTLs, five gliadin QTLs, six glutenin QTLs) based on starch content. Of these QTLs, QAlu1B, QGlo6A, QGli1B, QGli7A, QGlu1B and QGlu1D increased the protein-fraction contents independent of the starch content. These QTLs could regulate the usual inverse relationship between protein and starch in wheat seeds. The results could possibly be used in the simultaneous improvement of grain protein and starch content in wheat breeding.
机译:蛋白质和淀粉对小麦的品质和产量至关重要。为了了解定量性状基因座(QTL)/基因水平上蛋白质和淀粉之间的遗传关系,在两年内的三个位置使用了168个双倍单倍体(DH)系。通过无条件和有条件的QTL作图分析了蛋白质馏分含量和淀粉含量的QTL。我们检测到17个无条件添加QTL(四个白蛋白QTL,三个球蛋白QTL,六个麦醇溶蛋白QTL,四个谷蛋白QTL)来控制蛋白质组分含量。我们根据淀粉含量检测到19个条件性QTL(五个白蛋白QTL,三个球蛋白QTL,五个麦醇溶蛋白QTL,六个谷蛋白QTL)。在这些QTL中,QAlu1B,QGlo6A,QGli1B,QGli7A,QGlu1B和QGlu1D增加了蛋白质级分含量,而与淀粉含量无关。这些QTL可以调节小麦种子中蛋白质与淀粉之间通常的逆向关系。该结果可能有助于小麦育种同时提高籽粒蛋白质和淀粉含量。

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